Journal: Arthritis Research & Therapy

Article Title: Expansion of myeloid-derived suppressor cells contributes to metabolic osteoarthritis through subchondral bone remodeling

doi: 10.1186/s13075-021-02663-z

Figure Lengend Snippet: High-fat diet increases monocytic-MDSC expansion during PTOA. A Gating strategy used to define the M-MDSC subpopulation (CD11b + Ly6C + Ly6G − cells) in mouse bone marrow. Following the initial FS/SC discrimination, the gate was set on CD11b + cells. After exclusion of doublets (not shown), live CD11b + cells were gated and Ly6C + and Ly6G + populations. B Cytospin images of M-MDSC cells from the bone marrow from LFD and HFD mice (at 16 weeks). Scale bar denotes 10 microns. C HFD increases the BM M-MDSC population in mice ( n = 4 in each group) by total number and percentage with concomitant suppression of CD8 + and CD4 + T-cells compared to LFD mice ( n = 6 in each group, at 16 weeks). D HFD increases the BM M-MDSC population in DMM mice ( n = 3 in each group). * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 by two-way ANOVA Tukey’s multiple comparison test

Article Snippet: The following anti-human antibodies were purchased from Miltenyi Biotec (Germany): CD11b-APC (clone M1/70, # 130-113-793), HLA-DR–PE (clone AC122, #130-113-402), CD14-fluorescein isothiocyanate (FITC) (clone Tuk4, #130-113-146), CD15-APCvio770 (clone VIMC6, #130-104-992), CD33-viobright515 (clone REA775, #130-111-027), CD66b-PEvio770 (cloneREA306, #130-119-808), and their corresponding isotype controls, along with corresponding isotype controls.

Techniques: Comparison

Journal: Arthritis Research & Therapy

Article Title: Expansion of myeloid-derived suppressor cells contributes to metabolic osteoarthritis through subchondral bone remodeling

doi: 10.1186/s13075-021-02663-z

Figure Lengend Snippet: MDSCs are expanded in obese patients with post-traumatic osteoarthritis. A Demographic of the patient population. B Gating strategy used to define the M-MDSC subpopulation (CD11b + HLADR − CD14 + CD15 − cells). The M-MDSC population is expanded in C peripheral blood and D synovial fluid of obese ( BMI > 30; N = 10) compared to non-obese ( BMI < 30; N = 5) patients diagnosed with post-traumatic osteoarthritis. Two-tailed unpaired Student t -test

Article Snippet: The following anti-human antibodies were purchased from Miltenyi Biotec (Germany): CD11b-APC (clone M1/70, # 130-113-793), HLA-DR–PE (clone AC122, #130-113-402), CD14-fluorescein isothiocyanate (FITC) (clone Tuk4, #130-113-146), CD15-APCvio770 (clone VIMC6, #130-104-992), CD33-viobright515 (clone REA775, #130-111-027), CD66b-PEvio770 (cloneREA306, #130-119-808), and their corresponding isotype controls, along with corresponding isotype controls.

Techniques: Two Tailed Test

Journal: Journal of Neuroscience

Article Title: Suppression of Alzheimer-Associated Inflammation by Microglial Prostaglandin-E2 EP4 Receptor Signaling

doi: 10.1523/jneurosci.0410-14.2014

Figure Lengend Snippet: Figure 5. EP4 receptor conditional knock-out in microglia enhances inflammation and amyloid deposition early at 5 months in APPSwe-PS1E9 (APPS) mice. Microglial EP4 receptor conditional knock-out (EP4-cKO) 5 month female and 9 month male APPS or nontransgenic (Non-tg) mice were generated using the CD11b-Cre; EP4 lox/lox strategy. A, qRT-PCR from hippocampal RNA demonstrates elevated CCL3 expression in APPS EP4-cKO mice at 5 months but not 9 months, and a trend toward increased IL-1 in 5 month APPS-EP4cKO mice. B, LC/ESI/MS/MS analysis of cortical lysates demonstrates elevated -ketoaldehyde adduct levels in young APPS EP4-cKO mice at 5 months. A40 (C) and (D) A42 ELISAs from guanidine-extracted cerebral cortex demonstrate increased A levels in EP4-cKO mice in 5 month but not 9 month APPS-EP4cKO mice. E, Immunostaining for 6E10-positive A plaques (arrows) and quantification of A plaque area in the hippocampus (F) demonstrates increased A plaque area and density in APPS EP4-cKO mice at 5 months of age. G, Congo Red staining for plaque cores (white arrows) and quantification of Congo Red-positive area in the hippocampus and cerebral cortex (H) demonstrates increased dense-core plaque percentage area in APPS EP4-cKO mice (A–H; n 4–11 mice per group). *p 0.05 (unpaired t test). **p 0.01 (unpaired t test). ***p 0.0001 (unpaired t test). I, J, Quantitative Western analysis of PSD-95 demonstrates significant decrease in 5 month (n 4/genotype; p 0.05) and in 9 month cohorts (n 3–5 mice per genotype; ANOVA p 0.032, post hoc Bonferroni multiple comparison. *p 0.05, APP-PS1;Cd11bCre; EP4 / versus APP-PS1;Cd11Cre;EP4 lox/lox, with conditional deletion of EP4 in APP-PS1 mice.

Article Snippet: Immunocytochemistry for mouse EP4 was performed using a chicken antibody directed against the mouse EP4 receptor (1:500), described and validated in (Liang et al., 2011), and a rat monoclonal antibody directed against mouse CD11b (1:500, AbD Serotec).

Techniques: Knock-Out, Generated, Quantitative RT-PCR, Expressing, Tandem Mass Spectroscopy, Immunostaining, Staining, Western Blot, Comparison

Journal: BMC Molecular and Cell Biology

Article Title: Transcriptome and proteome profiling reveal complementary scavenger and immune features of rat liver sinusoidal endothelial cells and liver macrophages

doi: 10.1186/s12860-020-00331-9

Figure Lengend Snippet: Antibodies used in the study

Article Snippet: CD11b/c Biotin (OX-42) , CD11b/c, CR3 , Cedarlane , CL042B , 2 μg/ml.

Techniques: Concentration Assay, Flow Cytometry, Control, Staining, FACS

Journal: STAR Protocols

Article Title: Isolation of mouse brain-infiltrating leukocytes for single cell profiling of epitopes and transcriptomes

doi: 10.1016/j.xpro.2021.100537

Figure Lengend Snippet:

Article Snippet: CyTOF: rat anti-mouse CD11b-148Nd (M1/70) , Fluidigm , Cat#3148003B; RRID: AB_2814738.

Techniques: Recombinant, Staining, Blocking Assay, Saline, Multiplex Assay, Software

Journal: STAR Protocols

Article Title: Isolation of mouse brain-infiltrating leukocytes for single cell profiling of epitopes and transcriptomes

doi: 10.1016/j.xpro.2021.100537

Figure Lengend Snippet: CyTOF Antibody Volumes (per 50 μL Maxpar Cell Staining Buffer/sample)

Article Snippet: CyTOF: rat anti-mouse CD11b-148Nd (M1/70) , Fluidigm , Cat#3148003B; RRID: AB_2814738.

Techniques: Staining

Journal: Cell Reports Medicine

Article Title: Selective apoptosis of tumor-associated platelets boosts the anti-metastatic potency of PD-1 blockade therapy

doi: 10.1016/j.xcrm.2025.101984

Figure Lengend Snippet:

Article Snippet: PE/Cyanine5 anti-mouse/human CD11b (clone: M1/70) , Elabscience , CatE-AB-F1081G.

Techniques: Recombinant, Enzyme-linked Immunosorbent Assay, Software, Staining, Modification, Clinical Proteomics, Membrane